ETEKSI WSSV (WHITE SPOT SYNDROM VIRUS) PADA LOBSTER AIR TAWAR (PROCAMBARUS CLARKII) MENGGUNAKAN METODE REAL TIME-PCR

Nefi Andriana, Muhamad Ali, Sulaiman N. Depamede

Abstract


Serangan virus White Spot Syndrome (WSSV) telah memberikan dampak finansial yang cukup besar sejak tahun 1992. Tingkat mortalitas yang mencapai 100% menyebabkan banyak pembudidaya udang dan lobster menderita kerugian. Untuk mengatasi permasalahan tersebut sangat diperlukan informasi tentang cara penyebaran virus WSSV sehingga dapat diketahui solusi yang tepat untuk menghentikan penyebarannya pada tambak-tambak udang maupun lobster. Pada penelitian ini, Lobster Air Tawar yang dibudidayakan di BBI Aik Bukak dijadikan kontrol negatif (belum terinfeksi virus WSSV). Hasil penelitian ini menunjukkan bahwa penularan virus WSSV pada lobster air tawar dapat berlangsung melalui konsumsi udang yang telah terinfeksi virus WSSV serta melalui aliran air. Pleopod atau kaki renang yang dideteksi dengan teknologi Real Time Polimerase Chain Reaction (RT-PCR) mengandung virus WSSV yang lebih dominan dibandingkan insang

Kata kunci: White Spot Syndrome Virus, Lobster Air Tawar, RT-PCR


Full Text:

PDF

References


Chang, P.S., Chen, H.C., and Wang, Y.C. 1998.Detection of White Spot SyndromeAsociated Baculovirus in Eksperimentally Infected Wild Shrimp, crab andLobsters by inSitu Hybridization Aquacult.164233-242.

Dieffenbach, C.W., T.M. J.Lowe, & G.S. Dveksler.1993. General Concepts for PCR Primer Design. PCR Methods Application 3:30-37.

Fatimi, Humairah. 2010. Polymerase Chain Reaction (PCR). Palembang : Program Studi Ilmu Biomedik Program Pasca Sarjana, Universitas Brawijaya.

Irawati.2008, Penambahan ekstrak wortel (Daucus carrota L) pada pakan untuk meningkatkan pertumbuhan dan tingkat kelangsungan hidup lobster air tawar(Cherax quadricarinatus).Thesis. Universitas Erlangga. Surabaya

Kasornchandra J, Boonyaratpalin S, Itami T (1998) Detection of white-spot syndrome in cultured penaeid shrimp in Asia: microscopic observation and polymerase chain reaction. Aquaculture 164:243–251

Kiiyuki, C. 2003. Laboratory Manual of Food Microbiology for Ethiopian Health and Nutrition Reasearch Institute (Food Microbiology Laboratory). Unido Project: 28-33, 75-80

Koesharyani isti, Tatik Mufidah, Hambali Supriyadi, Hessy Novita , 2008, Infeksi Infectious Myonecrosis Virus (IMNV) Pada Budidaya Udang Vaname (penaeus Vannamei) Di Jawa Timur.: 945-949

Lightner, D.V. 1996a.A Handbook of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultured penaeid Shrimp. The World Aquaculture Society. Baton Rouge, Louisiana, 70803 USA.

Lightner, D.V. 1996b.Epizootiology, distribution and the impact on international trade of two penaeid shrimp viruses in the Americas. Revue Scientifique et Technique Office International des Epizooties 15: 579-601.

Lio-Po, G.D., Albright, L.I., Traxler, G.S. and Leano, E.M. 2001.Pathogenicity of The Epizooeic Ulcerative Syndrome (EUS)-Associated Rhabdovirus to Snakehead Ophicephalus Striatus. Fish Pathology 36, 57-66.

Lo C.F., Ho C.H., Peng S.E., Chen C.H. and 7 others. 1996. White Spot Syndrome Baculovirus (WSBV) Detected in Cultured and Captured Shrimp, Crabs and Other Arthropods. Dis Aquat Org 27:215–225

Lorenz, T. 1998. A Review of Carotenoid, Astaxanthin, as a Pigment and Vitamin Source for Cultured Penaeus Prawn. http://www.cyanotech.com. 7 hal.

_________. 2000. Natu Rose TM Natural Astaxanthin as a Carotenoid and Vitamin Source for Ornamental Fish and Animals. http://www.cyanotech.com. 8 hal.

Madeali, M. I., A. Tompo dan Muliani. 1998. Diagnosis Penyakit Viral Pada Udang Windu, Penaeus monodon Secara Histopatologis dan Antibody Poliklonal Dengan Metode Elisa. Jurnal Penelitian Perikanan Indonesia, 4 (3): 11-18.

Mahardika, K., Zafran dan I. Koesharyani. 2004. Deteksi White Spot Syndrome Virus (WSSV) Pada Udang Windu (Penaeus monodon) di Bali dan JawaTimur Menggunakan Metode Polymerase Chain Reaction (PCR). JurnalPenelitian Perikanan Indonesia, 10 (1): 55-60.

Martens, T.R. & R.L. Hammersmith. 1995. Genetics Laboratory Investigation. 10th ed. Prentice-Hall, Englewood Cliffs: vii + 277 hlm.

Sánchez-Paz, A., 2010. White spot syndrome virus: an overview on an emergent concern. Veterinary Research, 41, 43.

Pestana, E.A., S. Belak., A. Diallo., J.R. Crowther., G.J. Viljoen. 2010. Early Rapid and Sensitive Veterinary Moleculer Diagnostics – Real Time PCR Applications. Springer. Dordrecht Heidelberg, London New York

Pranawaty, R.N., Buwono, I.D., dan Liviawaty, E. 2012.Aplikasi Polymerase Chain Reaction (PCR) Konvensional dan Real Time PCR Untuk Deteksi White Spot Syndrome Virus Pada Kepiting.Jurnal Perikanan dan Kelautan. Vol. 3(4):61-74.

Prasetyo, A., 2009. Materi Asistensi Biomedik FK UNS. FK UNS. Semarang

Prijanto, Muljati. 1992. Polymerase Chain Reaction (PCR) untuk Diagnosis Human Immunodeficiency Virus (HIV). http://www.pcr.htm. Diakses 1 Mei 2014.

Polanski, A. & M. Kimmel. 2007. Bioinformatics. Springer, Berlin: xvii + 376 hlm.

Sambrook, J. & D.W. Russell. 2001. Molecular Cloning: A Laboratory Manual. 3rd ed. CSHL Press, New York: xxvii + 18.

Tonsakun, F., Siwaporn, L., S. Rukpratarnporn, P. Chaivisuthangkura, W. Sithigornul, and P. Sithigorngul. 2006. Experimental Infection of Three PalaemonidShrimps With White

Wang, Y. G., M. Shariff, P.M. Sudha, P.S. Srinivasa Rao, M.D. Hassan and L.T. Tan. 1998. Managing white spot disease in shrimp, Infofish International.p : 30-36.

Wang, C. S., Y. J. Tsai, G. H. Kou and S. N. Chen. 1997. Detection of White SpotSyndrome Disease Virus Infection in Wild Caught Greasyback Shrimp, Metapenaeus Ensis (deHaan) in Taiwan. Fish Pathology, 32 (1): 35-41.

Weeden, N. F., Timmerman, G. M., Hemmat, M., Kneen, B. E., dan Lodhi, M. A. 1992.Inheritance and reliability of RAPD markers.In Application of RAPD technology to plant breeding, symposium Proceedings, Crop Science Of America, Madison, W. I., p.12-17.

Wijayati, A., dan N. Fahris. 1999. Teknik Deteksi Serangan Virus. Pelatihan Petugas Pengamat Penyakit. Balai Besar Pengembangan Budidaya Air Payau (BBPBAP).Jepara.8 hlm.

Yuwono, T. 2006. Teori dan Aplikasi Polymerase Chain Reaction. Penerbit Andi: Yogyakarta. Spot Syndrome Virus (WSSV).Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok.




DOI: https://doi.org/10.29303/jstl.v1i1.10

Article Metrics

Abstract view : 376 times | PDF - 90 times

DOI (PDF): https://doi.org/10.29303/jstl.v1i1.10.g8

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Sains Teknologi dan Lingkungan (JSTL)

(ISSN Print: 2477-0329, ISSN Online: 2477-0310)

Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Mataram