Karakter Morfologi dan Identifikasi Molekuler dengan Menggunakan Marka Gen 12S rRNA pada Ikan Baronang (Siganus spp.) di Perairan Laut Selatan Pulau Lombok

Mahrus Mahrus, Abdul Syukur

Abstract


This study aims to obtain the certainty of the number of species of baronang fish (Siganus spp.) Siganidae family in the waters of the south sea of Lombok Island.  This study used a sample of 90 fish from the fishermen's catch. Sampling used a random method three times. Observation of morphological characters used 90 and nine fishes for molecular characters-12S rRNA genes. The study used a descriptive technique for data analysis. The results showed that the fish is only one species and suspected consists of 3 variants. Three variants of fish have the same 12S rRNA genes with a fragment size of approximately 1000 bp. The morphological classification of the three groups of fish does not indicate the number of species, but no more as genetic variants.

Keywords


baronang fish; morphology;12S gene; genetic variants; PCR

Full Text:

PDF

References


Ariyanti, Y., and Sianturi, S. 2019. Ekstraksi DNA total dari sumber jaringan hewan (Ikan Kerapu) menggunakan metode kit for animal tissue. Journal of Science and Applicative Technology, 3(1), 40-45.

Baaka, A. 2013. Kajian keragaman genetik gen Cytochrome Oxidase SubUnit 1 (COX1) Tarsius sp. sebagai upaya penentuan asal usul Tarsius sp (Doctoral dissertation, Universitas Gadjah Mada).

Barr, N.B., Copeland, R.S., De Meyer, M., Masiga, D., Kibogo, H.G., Billah, M.K., Osir, E., Wharton, R.A., and McPheron, B.A. 2006. Diagnostik molekuler ekonomis penting spesies lalat buah Ceratitis (Diptera: Tephritidae) di Afrika menggunakan analisis PCR dan RFLP. Bull Entomol Res., 96: 505-521.

Bray, R. A., and Cribb, T. H. 2001. Lecithophyllum kitrii n. sp.(Digenea: Lecithasteridae) from Australian coral reef fishes of the genus Siganus. Systematic parasitology, 50(2), 143-147.

Burhanuddin, A. I., Budimawan and Sahabuddin. 2014. The Rabbit-Fishes (Family Siganidae) from The Coast of Sulawesi, Indonesia. International Journal of plant, animal and environmental sciences, 4(4): 95-102.

Calder, P. C. 2012. The role of marine omega-3 (n-3) fatty acids in inflammatory processes, atherosclerosis and plaque stability. Mol Nutr Food Res 56:1073–1080

Dalmasso, A., Fontanella, E., Piatti, P., Civera, T., Rosati, S., and Bottero, M. T. 2004. A multiplex PCR assay for the identification of animal species in feedstuffs. Molecular and cellular probes, 18(2), 81-87.

Ferri, G., Alù, M., Corradini, B., Licata, M., and Beduschi, G. 2009. Spesies identifikasi melalui DNA "barcode". Biomarker Genet Mol Test., 13: 421-6.

Ghufron. M., and Kordi, H. 2005. Budidaya Ikan Laut di Keramba Jaring Apung. Penerbit Rineka Cipta, Jakarta.

Gupta, A.R., Patra, R.C., Das, D.K., Gupta, P.K., Swarup, D., and Saini, M. 2008. Sequence karakterisasi dan polymerase chain reaction-pembatasan Polimorfisme panjang fragmen gen 12S rRNA DNA mitokondria menyediakan metode untuk identifikasi spesies rusa India. DNA mitokondria, 19: 394-400.

Hamka. 2006. Upaya Pematangan Gonad Ikan Baronang (Siganus guttatus) Melalui Pemberian Pakan Buatan dan Pakan Alami (Gracillaria sp.) pada Bak Terkontrol. Laporan Tahunan. Balai Budidaya Air Payau Takalar.

Hendrawan, I. G and Asai, K. 2011. Numerical Study of Tidal Upwelling Over The Sill In The Lombok Strait (Indonesia). In: Proceedings of the Twenty-first (2011) International Offshore and Polar Engineering Conference. Maui, Hawaii, USA: the International Society of Offshore and Polar Engineers (ISOPE); 949–956.

Hou, W., Y. Chen, X. Wu, J. Hu, Z. Peng, J. Yang, Z. Tang, C. Zhou, Y. Li, S. Yang, Y. Du, L. Kong, Z. Ren, H. Zhang and S. Shui. 2006. A Complete Mitochondrial Genome sequence of Asian Black bear Sichuan Subspecies (Ursus thibetanus mupinensis). Int. J. Biol. Sci. 3(2):85-90.

Ioja-Boldura, O. M., Popescu, S., Bruznican, S., and Hutu, I. 2011. Applicability of a multiplex PCR assay for the identification of animal species in feedstuffs. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies, 68(1-2).

Kuiter, R.H., and Tonozuka, T. 2001. Indonesian reef fishes. Part 2. Fusiliers to dragonets: Caesionidae to Callyonimidae. Zoonetic, Melbourne. Australia. 161p.

Kune, S. 2007. Pertumbuhan rumput laut yang dibudidaya bersama ikan Baronang. Jurnal Agrisistem, 3(1), 7-9.

Lam, T. J. 1974. Siganid. Their biology and mariculture potential. Aquaculture (3). Pp. 324-354.

Mahrus, Zulkifli, L., and Karnan. 2018. Keragaman genetik ikan baronang (Siganus spp.) berdasarkan gen 12S rRNA dari perairan laut selatan pulau Lombok. Prosiding Seminar Nasional Pertanian, Faperta Unram, Mataram, 29 November 2018.

Mahrus, Sumitro, S. B., Utomo, D. H., Sartimbul, A., Toha, A. H., and Widodo, N. 2012. Genetic relationship of Sardinella lemuru from Lombok Strait with fish rich in omega-3 fatty acid. Bioinformation, 8 (25): 1271-1276.

Marengo, M., Durieux, E. D., Marchand, B., and Francour, P. 2014. A review of biology, fisheries and population structure of Dentex dentex (Sparidae). Reviews in fish biology and fisheries, 24(4), 1065-1088.

Moritz, C. 1999. Conservation units and translocations: strategies for conserving evolutionary processes. Hereditas, 130(3), 217-228

Muhiddin, A. H. 2010. Identifikasi dan klasifikasi kawanan lemuru Selat Bali berdasarkan data hidroakustik dengan metode statistik. Torani (Jurnal Ilmu Kelautan dan Perikanan), 20 (1): 25-36.

Musaiger, A.O and D’Souza, R. 2011. Fatty acid profile of raw and cooked fish consumed in Bahrain. African Journal of Food Science, 5(4): 213 – 218.

Nabavi, S. F., Bilotto, S., Russo, G. L., Orhan, I. E., Habtemariam, S., Daglia, M., and Nabavi, S. M. (2015). Omega-3 polyunsaturated fatty acids and cancer: lessons learned from clinical trials. Cancer and Metastasis Reviews, 34(3), 359-380.

Prehadi, P., Sembiring, A., Kurniasih, E. M., Rahmad, R., arafat, D., Subhan, B., and Madduppa, H. H. 2015. DNA barcoding and phylogenetic reconstruction of shark species landed in Muncar fisheries landing site in comparison with Southern Java fishing port. Biodiversitas Journal of Biological Diversity, 16(1).

Randall, J. E., G. R., Allen, R. C., and Steene. 1997. Fishes of the Great Barrier Reef and Coral Sea. University of Hawaii Press, Honolulu, HI, 507 p.

Ratnasingham, S., and Hebert, P.D.N. 2007. BOLD: The Life Barcode Sistem Data. Mol Ecol., 7: 355-364.

Sahabuddin, I. Burhanuddin, A. C. Malina and Nurhapsa. 2015. Morfometrik dan meristik ikan baronang (Siganus canaliculatus Park, 1797) di perairan teluk Bone dan selat Makassar. Torani (Jurnal Ilmu Kelautan dan Perikanan), 25(1): 44-52.

Sakalar, E., and Abasiyanik, M. F. 2011. Qualitative analysis of meat and meat products by multiplex polymerase chain reaction (PCR) technique. African Journal of Biotechnology, 10(46), 9379-9386.

Samonte, I., Canlas, R., Alvia, K., Carvajal, T., and Pagulayan, R. 2009. Multivariate analyses of the biometric features from Philippine sardines-implications for the phylogenetic relationships of the freshwater Sardinella tawilis (Teleostei, Clupeomorpha). J Zool Syst Evol Res., 47(1): 21-24.

Sayanova, O., Haslam, R. P., Calerón, M. V., López, N. R., Worthy, C., Rooks, P., ... and Napier, J. A. 2011. Identification and functional characterisation of genes encoding the omega-3 polyunsaturated fatty acid biosynthetic pathway from the coccolithophore Emiliania huxleyi. Phytochemistry, 72(7), 594-600. Emiliania huxleyi. Phytochemistry, 72(7): 594–600.

Singh, D., Khullar, N., and Jha, C. 2015. Lucrative potentials of mitochondrial DNA: A laconic review accentuating particularly blow flies beyond forensic importance. Journal of Entomology and Zoology Studies, 3(4): 01-08.

Suseno, S.H., Tambunan, J.E., Ibrahim, B and Saraswati. 2014. Inventory and Characterization of Sardine (Sardinella sp.) Oil from Java Island-Indonesia. Advance Journal of Food Science and Technology, 6(5): 588-592.

Syukur, A., Mahrus. and Sychrudin . A. 2016. The potential assessment environment friendly aquaculture of small-scale fishermen as a conservation strategy seagrass beds in coastal areas of Tanjung Luar East Lombok, Indonesia.

Tang, K.L., Berendzen, ,P. B., Wiley, E. O., Morrissey, J. F., Winterbottom, R., and Johnson, G. D. 1999. The phylogenetic relationships of the suborder Acanthuroidei (Teleostei: Perciformes) based on molecular and morphological evidence. Mol. Phylogenet. Evol. 11 (3), 415-425. PUBMED 10196082.

Tarwijah. 2001. Pembenihan Ikan Baronang (Siganus sp). Booklet Jenis-Jenis Komoditi Laut Ekonomis Penting Pada Usaha Pembenihan. Direktorat Bina Pembenihan, Dirjen Perikanan, Departemen Pertanian, Jakarta.

Wong, K.L., Wang. J., Tapi, P.P.H., and Shaw, P.C. 2004. Aplikasi urutan DNA ctyochrome b untuk otentikasi spesies ular terancam punah. Forensic Sci Int., 139: 49-55.

Woodland, D. J and Anderson, R. C. 1990. Description of a new species of rabbitfish (Perciformes: Siganidae) from southern India, Sri Lanka and the Maldives. Zootaxa, 3811 (1): 129–136.

Woodland, D. J. 1990. Revision of the fish family Siganidae with descriptions of two new species and comments on distribution and biology. Indo-Pacific Fishes, 19.

Yusmalinda, N. L. A., Aji, W., Dio, M. S., I Made J. R., Dwi, S., Danielle, K,, and Ni Kadek,D. C. 2017. Identifikasi jenis pada kejadian cetacea terdampar di Indonesia dengan teknik molekuler. Jurnal Ilmu dan Teknologi Kelautan Tropis, 9 (2): 465-474




DOI: https://doi.org/10.29303/jstl.v6i1.156

Article Metrics

Abstract view : 30 times | PDF - 8 times

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Jurnal Sains Teknologi dan Lingkungan (JSTL)

(ISSN Print: 2477-0329, ISSN Online: 2477-0310)

Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Mataram